MSE PRO Mouse MaR1(Maresin 1) ELISA Kit

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SKU: EK0064929-96T
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MSE PRO™ Mouse MaR1(Maresin 1) ELISA Kit

Specifications:

Product name Mouse MaR1(Maresin 1) ELISA Kit
Reactivity Mouse
Alternative Names 7(R)-Maresin 1; quinolin-7-ol; 7-hydroxychinoline; (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-7,14-Dihydroxy-4,8,10,12,16,19-docosahexaenoic acid; 7-Chinolinol; 7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid; 4,8,10,12,16,19-Docosahexaenoic acid, 7,14-dihydroxy-, (4Z,7R,8E,10E,12Z,14S,16Z,19Z)-; 7-Quinolinol; 7-HYDROXYQUINOLINE; 7R-maresin-1; 7-hydoxyquinoline
Assay Type Competitive Inhibition
Sensitivity 6.06 pg/mL
Standard 1000 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Type serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length 2.5h
Research Area Immunology & Inflammation
Test principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MaR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve.