Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
Couldn't load pickup availability
Still have questions? Ask our experts!
Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
| Detection Range | 31.25-2000 pg/mL |
| Sensitivity | 18.75 pg/mL |
Product Details
Properties
| Assay type | Sandwich-CLIA |
| Format | 96T/48T |
| Assay time | 3.5h |
| Detection range | 31.25-2000 pg/mL |
| Sensitivity | 18.75 pg/mL |
| Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
| Specificity | This kit recognizes Human FABP3 in samples. No significant cross-reactivity or interference between Human FABP3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 15%. |
| Application | This CLIA kit applies to the in vitro quantitative determination of Human FABP3 concentrations in serum, plasma and other biological fluids. |
Test Principle
Kit Components & Storage
An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips |
-20℃, 12 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial |
|
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
|
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FABP3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FABP3 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 102.64 | 225.20 | 728.63 | 93.93 | 228.99 | 730.08 |
| Standard deviation | 9.67 | 15.76 | 83.57 | 11.67 | 23.70 | 78.63 |
| CV (%) | 9.42 | 7.00 | 11.47 | 12.42 | 10.35 | 10.77 |
Recovery
The recovery of Human FABP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-111 | 105 |
| EDTA plasma(n=8) | 98-115 | 105 |
| Cell culture media(n=8) | 98-113 | 104 |
Target Information
Assay Procedures
 |
1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. |
 |
2.Remove the liquid. |
 |
3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. |
 |
4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. |
 |
5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. |
 |
6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |