ZAP70 Polyclonal Antibody Reactivity Human
SKU: E-AB-52977-200
ZAP70 Polyclonal Antibody Reactivity Human
| SKU # | E-AB-52977 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Applications | WB, IHC |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human ZAP70 |
| Abbre | ZAP70 |
| Synonyms | 70 kDa zeta associated protein, 70 kDa zeta-associated protein, EC 2.7.10.2, FLJ17670, FLJ17679, SRK, STD, Selective T cell defect, Syk related tyrosine kinase, Syk-related tyrosine kinase, Truncated ZAP kinase, Tyrosine protein kinase ZAP70, Tyrosine-protein kinase |
| Swissprot | |
| Calculated MW | 70 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. |
| Concentration | 1.02 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Immunology, Signal Transduction, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:50-1:300 |
Background
Zeta-associated protein (ZAP)-70,a member of the Syk family of tyrosine kinases,plays an important role in T-cell receptor signaling,natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.