This enzyme is useful for enzymatic determination of urea in clinical analysis.
Tribioscience Urease, Enzyme Activity (TBP0044)
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Tribioscience Urease, Enzyme Activity (TBP0044)
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ(-201) 100U/mg-solid or more
Contaminants: Asparaginase ≤2.0×10⁻²%
Arginase ≤2.0×10⁻³%
NH₄⁺ ≤5.0×10⁻⁴µg/U
Stabilizers: EDTA, glutathione, succinate, BSA
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 480,000
Isoelectric point: 5.0-5.1
Michaelis constant: 1.05×10⁻²M (Urea)
Structure: 8 active sites with SH-groups per the enzyme molecule
Inhibitors: Heavy metal ions (Ag⁺,Hg⁺⁺,etc.)
Optimum pH: 6.0
Optimum temperature: 60°C
pH Stability: pH 5.5-8.5 (30°C, 17hr)
Thermal stability: below 50°C (pH 8.0, 60min)
Applications