Tribioscience Urease, Enzyme Activity (TBP0044)

Tribioscience Urease, Enzyme Activity (TBP0044)

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Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅡ(-201) 100U/mg-solid or more

Contaminants: Asparaginase ≤2.0×10⁻²%

Arginase ≤2.0×10⁻³%

NH₄⁺ ≤5.0×10⁻⁴µg/U

Stabilizers: EDTA, glutathione, succinate, BSA

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 480,000

Isoelectric point: 5.0-5.1

Michaelis constant: 1.05×10⁻²M (Urea)

Structure: 8 active sites with SH-groups per the enzyme molecule

Inhibitors: Heavy metal ions (Ag⁺,Hg⁺⁺,etc.)

Optimum pH: 6.0

Optimum temperature: 60°C

pH Stability: pH 5.5-8.5 (30°C, 17hr)

Thermal stability: below 50°C (pH 8.0, 60min)

Applications