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The Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 0.5pg/mL, and finish in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 0.5 to 2048 pg/mL. The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.
For Research Use only
Simple Procedures
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human IL-33 Microplate | TBS3245A | 96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IL-33. | Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human IL-33 Standard | TBS3245B | 100 µL of Recombinant human IL-33 protein (100 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3245C | 2.2 ml of HRP-Human IL-33 antibody. | May be stored for up to 3 months at 2-8 °C. |
| Assay Diluent | TBS3245D | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.