Tribioscience Tribo™ Terrific Broth (TB) Medium

Tribioscience Tribo™ Terrific Broth (TB) Medium

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Tribo™ Terrific Broth (TB) Medium is a highly enriched medium developed to improve yields in recombinant E. coli. Due to the enriched formulation of TB, strains have an extended growth phase. TB contains Tryptone, yeast extract, potassium phosphate, and glycerol.

Applications

Suitable for non-selective cultivation of E. coli strains for cloning, gene transformation, and production of recombinant proteins. Since this medium supports higher cell densities than LB medium, it is preferred for maximizing plasmid yield in preparation for nucleic acid purification. Also suitable for selective cultivation when appropriate antibiotics are added.

Key Features

• Filter-sterilized.
• Ready-to-use format.
• Convenient package size.
• Standard formulation.

Compositions

• 1.2% Tryptone.
• 2.4% Yeast Extract.
• 72mM Dipotassium Phosphate (K2P04)
• 17mM Monopotassium Phosphate (KH2P04)
• 0.4% Glycerol.
• pH: 7.0 ±0.2

Storage Conditions

The product can be stored for 1 year at 4-8℃.