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Tribioscience’s Fast Human AFP ELISA is designed to quantitatively detect human AFP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The hands-on time can be within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 10 to 1000 pg/mL. The levels of human AFP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human AFP protein.
Typical Data
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 10 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human AFP. No cross-reactivity with others.
For Research Use only
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Component | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human AFP Microplate | TBS3212A | 96 well strip microplate (12 strips of 8 wells) coated with a human AFP monoclonal antibody. | Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C. |
| Human AFP Standard | TBS3212B | 30 µL of Recombinant human AFP protein (40 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3212C | 2.2 mL of HRP-Human AFP antibody. | May be stored for up to 6 months at 2-8 °C. |
| Assay Diluent | TBS3212D | 15 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.