Tribioscience ß-Glucosidase, Enzyme Activity (TBP0007)

Tribioscience ß-Glucosidase, Enzyme Activity (TBP0007)

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Preparation and Specification

Appearance: Light yellow amorphous powder, lyophilized

Activity: GradeⅠ 15U/mg-solid or more (containing approx. 30% of BSA)

Contaminant: α-Amylase ≤5.0×10⁻⁴%

Stabilizers: BSA, glutathione (reduced)

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 110,000

Isoelectric point: 7.3

Michaelis constants: 2.8×10⁻³M (p-Nitrophenyl-ß-D-glucopyranoside), 3.3×10⁻³M

(2,4-Dichlorophenyl-ß-D-glucopyranoside)

Structure: 2 subunits per enzyme molecule

Optimum pH: 5.5

Optimum temperature: 50-55°C

pH Stability: pH 6.0-9.0 (25°C, 64hr)

Thermal stability: below 50°C (pH 7.3, 1hr)

Applications

This enzyme is useful for structural investigations of carbohydrates and for the enzymatic determination of α-amylase in combination with α-glucosidase in clinical analysis.