Tribioscience ß-Glucosidase, Enzyme Activity (TBP0007)
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Tribioscience ß-Glucosidase, Enzyme Activity (TBP0007)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Light yellow amorphous powder, lyophilized
Activity: GradeⅠ 15U/mg-solid or more (containing approx. 30% of BSA)
Contaminant: α-Amylase ≤5.0×10⁻⁴%
Stabilizers: BSA, glutathione (reduced)
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 110,000
Isoelectric point: 7.3
Michaelis constants: 2.8×10⁻³M (p-Nitrophenyl-ß-D-glucopyranoside), 3.3×10⁻³M
(2,4-Dichlorophenyl-ß-D-glucopyranoside)
Structure: 2 subunits per enzyme molecule
Optimum pH: 5.5
Optimum temperature: 50-55°C
pH Stability: pH 6.0-9.0 (25°C, 64hr)
Thermal stability: below 50°C (pH 7.3, 1hr)
Applications
This enzyme is useful for structural investigations of carbohydrates and for the enzymatic determination of α-amylase in combination with α-glucosidase in clinical analysis.