This enzyme is useful for the determination of amino acid sequences of peptides and proteins containing proline residues.
Tribioscience Proline specific endopeptidase, Enzyme Activity (TBP0040)
SKU: TBP0040-50U
Tribioscience Proline specific endopeptidase, Enzyme Activity (TBP0040)
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Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅠ 5.0U/mg-solid or more
Contaminants: Leucine aminopeptidase ≤1.0×10⁻¹%
Trypsin-like activity ≤1.0×10⁻¹%
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 78,000
Isoelectric point: 9.1
Michaelis constants: 2.5×10⁻⁵M (Z-Gly-Pro-MCA), 1.4×10⁻⁴M (Z-Gly-Pro-2NNap)
Structure: Monomer
Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH₂Cl
Optimum pH: 6.5
Optimum temperature: 37°C (40°C)
pH Stability: 5.5-8.5 (30°C,15hr)
Thermal stability: below 40°C (pH7.0,10min)
Applications