Product Details
Formal Name: 2,6-Diaminopyridine-3,5-bis(thiocyanate)
Alternate Names: 3,5-Dithiocyanatopyridine-2,6-diamine
Molecular Formula: C7H5N5S2
Formula Weight: 223.28
CAS Number: 2645-32-1
Purity: >98%
Formulation: powder
Solubility: Soluble in DMSO (up to 5 mg/ml).
Storage: -20°C
Stability: ≥ 2 years.
Applications
DUB inhibitor
Functions
PR-619 is a nonselective, reversible inhibitor of cysteine-reactive deubiquitinating enzymes (DUBs; 5-20 µM). PR-619 is highly useful for preserving ubiquitinated proteins during cell lysis. DUBs become unregulated in cell lysates and will act quickly to deubiquitinate proteins of interest during cell lysis. To preserve maximum amounts of ubiquitinated protein, include PR-619 (50-100 µM) in lysis buffers. PR-619 is also useful for interrogating the role of DUBs in intact cell systems, and treatment of cells results in an overall accumulation of ubiquitinated proteins and many other phenotypes.
Application Procedures
First dissolved in DMSO (up to 5 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20° for up to 1 month.