This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. ⁵˜⁷⁾ For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Tribioscience N-Acetylneuraminic acid aldolase, Enzyme Activity (TBP0046)
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Tribioscience N-Acetylneuraminic acid aldolase, Enzyme Activity (TBP0046)
Research Use Only. Not intended for human use.
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Preparation and Specification
Appearance: Yellowish amorphous powder, lyophilized
Activity: Grade III 15U/mg-solid or more (30U/mg-protein or more) (containing approx. 30% of stabilizers)
Contaminants: Catalase ≤1.0%
NADH oxidase ≤1.0×10⁻³%
Stabilizers: Mannitol, EDTA
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 98,000
Isoelectric point: 4.6±0.1
Michaelis constant: 2.5×10⁻³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per enzyme molecule
Inhibitors: p-Chloromercuribenzoate, SDS, Hg⁺⁺, Ag⁺
Optimum pH: 7.5-8.0
Optimum temperature: 70°C
pH Stability: pH 6.0−9.0 (10°C, 25hr)
Thermal stability: below 65°C (pH 7.5, 30min)
Applications