Buy Tribioscience products from MSE Supplies at the best value.
Tribioscience’s Fast Mouse Glucagon ELISA is designed to quantitatively detect mouse glucagon levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of mouse glucagon samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse glucagon protein.
Typical Data
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of mouse glucagon is typically 13 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant mouse glucagon.
For Research Use only
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Mouse Glucagon Microplate | TBS3099A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse glucagon. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Mouse Glucagon Standard | TBS3099B | 30 µL of Recombinant mouse glucagon protein (100 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3099C | 2.2 mL of Biotin-mouse glucagon antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3099D | 300 µL of Streptavidin-HRP. | |
| Assay Diluent | TBS3099E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
The kit contains sufficient materials to run an ELISA on one 96 well plate.