Tribioscience ML210, GPX4 inhibitor; Ferroptosis Inducer (TBI4002)

Product Detail

Formal Name: (4-(Bis(4-chlorophenyl)methyl)piperazine-1-yl)(5-methyl-4-nitroisoxazol-3-yl)methanone      

Molecular Formula: C₂₂H₂₀Cl₂N₄O₄

Formula Weight: 475.3

CAS Number: 1360705-96-9

Purity: >98%

Formulation: Powder

Solubility: Soluble in DMSO (up to 30 mg/ml)

Storage: RT

Stability: ≥ 1 year.

Applications

GPX4 inhibitor; Ferroptosis Inducer

Functions

ML210 inhibits glutathione peroxidase 4 (GPX4), an important selenoenzyme that protects cells from ferroptosis caused by iron-catalyzed formation of free radicals from lipid peroxides. Exposure of several treatment-resistant cancer cell lines exhibiting a high mesenchymal state to ML210 resulted in selective induction of ferroptosis. ML210 is selectively lethal to HRAS^g12v expressing cells (IC₅₀ = 7.1 nM for BJeLR cells) compared to isogenic cells without HRAS^g12v (IC₅₀ = 272 nM for BJeH-LT cells). LUHMES dopaminergic neurons are highly susceptible to ML210-induced ferroptosis.

Application Procedures

First dissolved in DMSO (up to 30 mg/ml), then diluted to aqueous buffer. Solutions in DMSO may be stored at -20°C for up to 3 months.