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Tribioscience’s Mitochondrial Membrane Potential Assay is based upon JC-10 dye, which is a superior alternative to JC-1 dye. JC-10 dye is a cationic, lipophilic dye that gets concentrated in healthy mitochondria to form reversible red-fluorescent JC-10 aggregates (Ex/Em=540/590nm). In apoptotic cells, the mitochondrial membrane potential collapses, which results in failure to retain JC-10 dye and the dye returns to its monomeric green form (EX/Em= 490/525 nm) in the cytosol.
The kit, not only measures mitochondrial membrane potential, but can be used for monitoring apoptosis and for screening of apoptotic activators and inhibitors.
Storage Conditions
Store the unopened kit at -20 ℃ stable for 12 months. Do not use past kit expiration date.