This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5〜3.0 units of the enzyme per test (3.0ml) at pH around 7.0.
Tribioscience Lipoprotein lipase, Enzyme Activity (TBP0034)
SKU: TBP0034-10MG
Tribioscience Lipoprotein lipase, Enzyme Activity (TBP0034)
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Preparation and Specification
Appearance: Light brown amorphous powder, lyophilized
Activity: GradeⅢ 20U/mg-solid or more (containing approx. 80% of stabilizers)
Contaminants: Phosphatase ≤1.0×10⁻³%
Catalase ≤2.0×10⁻²%
NADH oxidase≤1.0×10⁻³%
Cholesterol oxidase ≤2.0×10⁻³%
Stabilizers: Mg⁺⁺, Na-cholate, BSA
Properties
Stability: Stable at -20°C for at least Two years
Molecular weight: approx. 134,000
Isoelectric point: 5.95±0.05
Inhibitors: Hg⁺⁺, Ag⁺, ionic detergents
Optimum pH: 7.0-9.0
Optimum temperature: 45-50°C
pH Stability: pH 7.0-9.0 (25°C, 20hr)
Thermal stability: below 55°C (pH 7.0, 10min)
Applications