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The TribioTM Human IA-2A ELISA is designed to quantitatively detect Human IA-2A levels in different tissues including tissue, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 31.2 to 2000 pg/mL. The levels of human IA-2A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IA-2A protein.
Typical Data
This standard curve (R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human IA-2A is typically 10 pg/ml.
The Intra-assay CV is 3.79%, the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human IA-2A.
For Research Use only
Simple Procedures
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human IA-2A Microplate | TBS3253A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IA-2A. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human IA-2A Standard | TBS3253B | 50 µl of Recombinant human IA-2A protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3253C | 2.1 ml of Biotin-Human IA-2A antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3253D | 300 µl of Streptavidin-HRP. | |
| Assay Diluent | TBS3253E | 25 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.