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Interleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.
Typical Data
This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sensitivity
The minimum detectable dose (MOD) of human IL-12/IL-23 p40 is typically 5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
Specificity
This assay recognizes natural and recombinant human IL-12/IL-23 P40.
No-cross reactivity:
Recombinant mouse IL-12; Recombinant mouse IL-12/IL-23 p40.
For Research Use only
Simple Procedures
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Human IL-12 Microplate | TBS3269A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12/IL-23 p40. | The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Human IL-12 Standard | TBS3269B | 30 μL of Recombinant human IL-12/IL-23 p40 protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3269C | 2.1 mL of human IL-12/IL-23 p40 antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3269D | 12 mL of Streptavidin-HRP | |
| Assay Diluent | TBS3269E | 12 mL of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |