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Tribioscience’s Fast Human γ-H2AX ELISA is designed to quantitatively detect human γ-H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 312.5 to 20000 pg/mL. The levels of human γ-H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human γ-H2AX protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 10 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay specifically recognizes Histone H2AX phosphorylated at S139.
No cross-reactivity with unphosphorylated recombinant human (rh) Histone H2AX, rhHistone Macro H2A1, and rhHistone Macro H2A2.
For Research Use only
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human γ-H2AX Microplate | TBS3265A | 96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human γ-H2AX. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human γ-H2AX Standard | TBS3265B | 100 µl of Recombinant human γ-H2AX (200 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3265C | 2.1 ml of biotin- human γ-H2AX antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3265D | 230 µl of streptavidin HRP (60x). | |
| Assay Diluent | TBS3265E | 25 ml of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.