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Tribioscience’s Fast ACTH ELISA is designed to quantitatively detect ACTH levels in serum, plasma, and other biological samples with our FAST proprietary approaches to combine samples and detections into a one-step. It makes the assay simple, easy, accurate, and fast. The total operating time can be finished within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 7 to 500 pg/mL. The ACTH levels of samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for ATCH.
Typical Data
This standard curve(R2=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of ACTH is typically 5 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human ACTH. No cross-reactivity with others.
For Research Use only
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human ACTH Microplate | TBS3290A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for ACTH. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human ACTH Standard | TBS3290B | 60 µl of Recombinant ACTH (10 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3290C | 2.1 ml of ACTH Detection antibody. | May be stored for up to 3 months at 2-8 °C. |
| Assay Diluent | TBS3290D | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.