Endotoxin-Free Utrapure Plasmid Extraction Maxiprep is composed of macroporous silica–based ion-exchange resin with a high density of diethylaminoethyl (DEAE) group, purification is based on the interaction between negatively charged phosphates of nucleic acids and positively charged DEAE groups on the surface of the resin, which could efficiently exclude all impurity during the binding-washing-elution process and yield ultrapure plasmid DNA.
Our powerful endotoxin-removal reagent EF Reagent can effectively remove lipopolysaccharides from bacterial cell lysates by phase partition without chilling step. Simply add the EF reagent into the clear cell lysate, and the lipopolysaccharide contaminants can be removed with the flow-through. The Kit System consistently produces endotoxin level less than 0.1 EU/μg DNA of endotoxin-free plasmid DNA product.
| Parameter | Value |
| DNA Fragment length | 1.5-kbp ⁓ 150 kbp |
| Maximal Capacity | >500µg |
| Maximal Recovery | 99% |
| Minimal Elution Volume | 10 mL |
| Regular Sample Volume | 100 mL |
| Endotoxin Level | Less Than 0.1EU/ μg |
Features
- Ion-exchange based columns for rapid and simple extraction.
- Reproducible yield of up to 500 µg of endotoxin-free plasmid DNA.
- Effective endotoxin removal reagent.
- Endotoxin level less than 0.1 EU/µg DNA in the final DNA product.
Application
- Transfection of sensitive cells (mammalian primary/suspension cells).
- Gene silencing study.
- Microinjection.
- NGS.
- Gene editing.