This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Tribioscience Cholesterol esterase, Enzyme Activity (TBP0012)
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Tribioscience Cholesterol esterase, Enzyme Activity (TBP0012)
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Preparation and Specification
Appearance: Light brown amorphous powder,lyophilized
Activity: GradeⅢ 100U/mg-solid or more (containing approx. 40% of stabilizers)
Contaminants: Catalase ≤1.0×10-2%
Stabilizers: Mg＋＋, Na-cholate, BSA
Properties
Stability: Stable at −20℃ for at least one year
Molecular weight: approx. 300,000
Isoelectric point: 5.9±0.1
Michaelis constants: 5.4×10-5M (Linoleate)
6.6×10-5M (Oleate)
3.7×10-5M (Linolenate)
1.5×10-4M (Palmitate)
1.2×10-4M (Myristate)
2.3×10-5M (Stearate)
Inhibitors: Hg＋＋, Ag＋, ionic detergents
Optimum pH: 7.0−9.0
Optimum temperature: 40℃
pH Stability: pH 5.0−9.0 (25℃, 24hr)
Thermal stability: below 55℃ (pH 7.5, 10min)
Applications