Tribioscience Cholesterol esterase, Enzyme Activity (TBP0012)

Tribioscience Cholesterol esterase, Enzyme Activity (TBP0012)

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Preparation and Specification

Appearance: Light brown amorphous powder,lyophilized

Activity: GradeⅢ　100U/mg-solid or more (containing approx. 40% of stabilizers)

Contaminants: Catalase ≤1.0×10^-2%

Stabilizers: Mg＋＋, Na-cholate, BSA

Properties

Stability: Stable at −20℃ for at least one year

Molecular weight: approx. 300,000

Isoelectric point: 5.9±0.1

Michaelis constants: 5.4×10^-5M (Linoleate)

6.6×10^-5M (Oleate)

3.7×10^-5M (Linolenate)

1.5×10^-4M (Palmitate)

1.2×10^-4M (Myristate)

2.3×10^-5M (Stearate)

Inhibitors: Hg^＋＋, Ag^＋, ionic detergents

Optimum pH: 7.0−9.0

Optimum temperature: 40℃

pH Stability: pH 5.0−9.0 (25℃, 24hr)

Thermal stability: below 55℃ (pH 7.5, 10min)

Applications