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The Canine IL-2 Fast ELISA is a solid phase ELISA designed to measure canine IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not 5-6 hours (Fig. 1). The detection range is from 62 to 4000 pg/mL. The levels of canine IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-2 protein.
Typical Data
This standard curve (R2=1) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of canine IL-2 is typically 62 pg/mL.
The Intra-CV is < 10%, the Inter-CV is < 10%.
Specificity
This assay recognizes natural and recombinant canine IL-2.
For Research Use only
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Canine IL-2 Microplate | TBS33002A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for Canine IL-2. | The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Canine IL-2 Standard | TBS33002B | 30 μL of Recombinant Canine IL-2 protein (200 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS33002C | 220 µL of Biotinylated Canine IL-2 antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS33002D | 300 µL of Streptavidin-HRP | |
| Assay Diluent | TBS33002E | 15 mL of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 15 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.