BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells. 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Tribioscience’s BrdU Cell Proliferation Colorimetric Assay Kit detects incorporated BrdU using an anti-BrdU antibody conjugate with Peroxidase (POD) and its substrate. The BrdU antibody POD conjugate allows direct detection of incorporated BrdU without the need for an additional secondary detection antibody. The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.
Method
- Blue color staining
Applications
Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents, and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.
Kit Components and Storage
| Part # | Name | Unit Size |
|---|---|---|
| A | 1000x Brdu Labeling Reagent | 30µL |
| B | 100x Anti-BrdU-POD | 300µL |
| C | Antibody Diluent | 20mL |
| D | 10x Washing Buffer | 25mL |
| E | FixDenat, Ready-to-use | 45mL |
| F | TMB Substrate | 25mL |
| Storage at –20℃. | ||
| Shelf-life: 6 months after receipt. | ||