Tribioscience Ascorbate oxidase, Enzyme Activity (TBP0006)

Tribioscience Ascorbate oxidase, Enzyme Activity (TBP0006)

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Preparation and Specification

Appearance: Light blue amorphous powder, lyophilized

Activity: GradeⅢ　200U/mg-solid or more (containing approx. 70% of stabilizers)

Contaminants: Catalase ≤1.0×10^-1%; Phosphatase ≤2.0×10^-2%

Stabilizers: BSA, borax, basic amino acids.

Properties

Stability: Stable at −20℃ for at least one year

Molecular weight: 132,000, 140,000

Isoelectric point: between 6.0 and 7.8, 8.2

Michaelis constant: 2.5×10^-4M (Ascorbate)

Structure: 8 copper atoms per enzyme molecule

Inhibitors: cyanide, Na₂S, diethyldithiocarbamate (Na)

Optimum pH: 5.6

Optimum temperature: approx. 30℃

pH Stability: pH 7.0−10.0 (25℃, 17hr)

Thermal stability: below 40℃ (pH 8.0, 30min)

Substrate specificity: This enzyme oxidizes ascorbic acid and several ascorbic derivatives.

Applications

This enzyme is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.