Alkaline phosphatase (AP) is a membrane bound enzyme synthesized by cells in many tissues, especially bone, liver, and stem cells. It hydrolyzes phosphate containing molecules under alkaline conditions. AP is widely used as a universal pluripotent marker to identify undifferentiated states for all types of pluripotent stem cells including embryonic stem cells, embryonic germ cells, and induced pluripotent stem cells. The undifferentiated state of stem cells can be characterized by a high level of AP expression, along with the expression of multiple pluripotency markers including the transcription factors Nanog, Oct4, Sox2, SSEA-1, SSEA-3/4, and tumor related antigens, TRA-1-60 and TRA-1-81.
Tribo™ Alkaline Phosphatase Staining Kit is a specific and sensitive tool for the phenotypic assessment of stem cell differentiation by the determination of AP expression.
Storage Conditions
Store all components at 4℃ for 6 months.