SMAD2 Polyclonal Antibody Reactivity Human
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SMAD2 Polyclonal Antibody Reactivity Human
| SKU # | E-AB-32916 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB, IHC-p |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Smad2 around the non-phosphorylation site of Ser250. |
| Abbre | SMAD2 |
| Synonyms | Drosophila, HsMAD2, JV18, JV18-1, JV181, MAD, MAD Related Protein 2, MAD homolog 2, MADH2, MADR2, MGC22139, MGC34440, Mad-related protein 2, Mother against DPP homolog 2, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 2, hMAD-2, homolog of |
| Swissprot | |
| Calculated MW | 52 kDa |
| Observed MW | 52 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
| IHC | 1:100-1:300 |
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.