RPMI-1640 (ATCC Modification)
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RPMI-1640 (ATCC Modification)
General Information
| Product Introduction | RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media. The ATCC-modified RPMI-1640 adds sodium pyruvate and HEPES to increase the sugar content and decrease the Sodium bicarbonate content. RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives. |
| Ingredient Statement |
With: 4500 mg/L D-Glucose |
| Concentration | 1 × |
| Format | Liquid |
| Bacterial Detection | Negative |
| Fungal Detection | Negative |
| Mycoplasmal Detection | Negative |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Endotoxin Level | ＜3 EU/mL |
| Storage | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping | Room Temperature |
| Expiration Date | 24 months |
Notes
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Frequently Asked Questions
How is ATCC-modified RPMI-1640 different from standard RPMI-1640?
It adds sodium pyruvate and HEPES, has more glucose (4500 mg/L) and less sodium bicarbonate (1500 mg/L).
Do I need to add serum?
Yes. The medium has no protein, lipids or growth factors, so use it with serum or serum-free additives.
What cells is it used for?
RPMI-1640 was designed for lymphocytes and is now widely used for normal and cancer cells, especially suspension cells.