Recombinant Swine IL-4 protein(N-His)(active)
SKU: PKSS000004-20
Recombinant Swine IL-4 protein(N-His)(active)
| SKU # | PKSS000004 |
| Expression Host | E.coli |
Description
| Synonyms | B-cell Stimulating Factor (BSF-1), BCDF, BCGF |
| Species | Porcine |
| Expression Host | E.coli |
| Sequence | His 25-Cys 133 |
| Accession | Q04745 |
| Calculated Molecular Weight | 13.5 kDa |
| Observed Molecular Weight | 15 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce proliferation in TF-1 cells.The ED50 for this effect is <0.6 ng/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation; survival and gene expression. IL-4 is produced by mast cells; T cells; and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by their cytokine-secretion profile that includes secretion of IL-4; IL-5; IL-6; IL-10; and IL-13; which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.