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Recombinant Rat IL-6/Interleukin-6 Protein (Active)
SKU: PKSR030420-100
Recombinant Rat IL-6/Interleukin-6 Protein (Active)
| SKU # | PKSR030420 |
| Expression Host | E.coli |
Description
| Synonyms | IL6 |
| Species | Rat |
| Expression Host | E.coli |
| Sequence | Phe25-Thr211 |
| Accession | NP_036721.1 |
| Calculated Molecular Weight | 21.9 kDa |
| Observed Molecular Weight | 22 kDa |
| Tag | None |
| Bio-activity | Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 1-6 ng/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 40% acetonitrile, 0.1% TFA Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.