Recombinant Mouse IGF-II protein(N-His)(active)
SKU: PKSM041521-100
Recombinant Mouse IGF-II protein(N-His)(active)
| SKU # | PKSM041521 |
| Expression Host | E.coli |
Description
| Synonyms | AI429687 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Ala 25-Glu 91 |
| Accession | P09535 |
| Calculated Molecular Weight | 8.2 kDa |
| Observed Molecular Weight | 11 kDa |
| Tag | N-His |
| Bio-activity | Measure by its ability to induce MCF-7 cells proliferation.The ED50 for this effect is <6 ng/mL.The specific activity of recombinant mouse IGF-II is > 1.5 x 105 IU/mg. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity. IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.