Recombinant Mouse Exostosin-Like 2/EXTL2 Protein (His Tag)
SKU: PKSM041014-50
Recombinant Mouse Exostosin-Like 2/EXTL2 Protein (His Tag)
| SKU # | PKSM041014 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 4-N-acetylhexosaminyltransferase EXTL2, Alpha-1, Alpha-GalNAcT EXTL2, EXT-related protein 2, Exostosin-like 2, Extl2, Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Asn43-Met330 |
| Accession | Q9ES89 |
| Calculated Molecular Weight | 33.6 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Exostosin-like 2 (EXTL2) is a member of the exostosin (EXT)-related family which contains five members: EXT1, EXT2, EXTL1, EXTL2, and EXTL3. Studies have shown that EXT gene family members have the activities of heparan sulfate-synthesizing glycosyltransferases. EXT1 and EXT2, which have been identified as causal genes for hereditary multiple exostoses, have HS-GlcAT-II and GlcNAcT-II activities. EXTL1 has GlcNAcT-II activity and EXTL3 has GlcNAcT-I and -II activities. EXTL2 has GlcNAcT-I and N-acetylgalactosaminyltransferase activities, and transfers a GlcNAc residue to the tetrasaccharide linkage region when this region is phosphorylated by a xylose kinase 1 (FAM20B) and thereby terminate chain elongation. In mice, lack of EXTL2 causes glycosaminoglycan (GAG) overproduction and structural changes of GAGs associated with pathological processes.