Recombinant Mouse CD64/FCGR1 Protein (His Tag)(Active)
SKU: PKSM040932-100
Recombinant Mouse CD64/FCGR1 Protein (His Tag)(Active)
| SKU # | PKSM040932 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD64, Fc-gamma RI, FcRI, FcgammaRI, High affinity immunoglobulin gamma Fc receptor I, IGGHAFC, IgG Fc receptor I |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro 297 |
| Accession | NP_034316.1 |
| Calculated Molecular Weight | 32.0 kDa |
| Observed Molecular Weight | 45-50 kDa |
| Tag | C-His |
| Bio-activity | Immobilized mouse CD64-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 μg/ml. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses..