Recombinant Mouse CD39/ENTPD1 Protein (His Tag)
SKU: PKSM041341-50
Recombinant Mouse CD39/ENTPD1 Protein (His Tag)
| SKU # | PKSM041341 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD39, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-ATPase 1, Ecto-apyrase, Ectonucleoside triphosphate diphosphohydrolase 1, Lymphoid cell activation antigen, NTPDase 1 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Thr38-Ile478 |
| Accession | P55772 |
| Calculated Molecular Weight | 50.5 kDa |
| Observed Molecular Weight | 60-90 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
Background
Ectonucleoside triphosphate diphosphohydrolase-1(NTPDase-1)is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1wasexpressed as a protein lacking its N- andC-terminaltransmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1was originally describedas CD39, a B lymphocyte cell surface marker. but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. NTPDase1hydrolyzes the β-andγ phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1plays arole in the regulation of purinergic signaling. NTPDase-1is involved in the processes of thromboregulation and vascular inflammation. The administration ofsoluble NTPDase-1may have therapeutic applications for the treatment of some vascular and transplantation-associateddiseases.