Recombinant Mouse Cathepsin D/CTSD Protein (His Tag)
SKU: PKSM041238-50
Recombinant Mouse Cathepsin D/CTSD Protein (His Tag)
| SKU # | PKSM041238 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CPSD, CTSD, Cathepsin D |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Ile21-Leu410 |
| Accession | NP_034113.1 |
| Calculated Molecular Weight | 43.9 kDa |
| Observed Molecular Weight | 56 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM MES,150mM NaCl,pH5.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CTSD localizes to the lysosome and consists of a light chain and a heavy chain. CTSD is expressed in epithelial cells as well as in macrophages.CTSD is a lysosomal aspartyl protease that depends critically on protonation of its active site Asp residue and gets activated at pH 5 in endosome of hepatocytes. It has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix and xonnective tissue. In addition, CTSD has been used as a breast cancer tumor marker.