Recombinant Mouse B7-H4/VTCN1 Protein (aa 25-256, Fc Tag)
SKU: PKSM040966-50
Recombinant Mouse B7-H4/VTCN1 Protein (aa 25-256, Fc Tag)
| SKU # | PKSM040966 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | B7 homolog 4, B7-H4, B7h4, B7s1, B7x, BC032925, Protein B7S1, V-set domain containing T-cell activation inhibitor 1, VTCN1 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Phe29-Pro258 |
| Accession | AAH32925.1 |
| Calculated Molecular Weight | 52.2 kDa |
| Observed Molecular Weight | 70-90 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Mouse V-set domain-containing T-cell activation inhibitor 1/VTCN1/B7-H4 is glycosylated member of the B7 family of immune co-stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.