Recombinant Mouse 4-1BB/TNFRSF9 Protein (aa 1-211, Fc Tag)(Active)
SKU: PKSM040474-100
Recombinant Mouse 4-1BB/TNFRSF9 Protein (aa 1-211, Fc Tag)(Active)
| SKU # | PKSM040474 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 4-1BB, A930040I11Rik, AA408498, AI325004, CDw137, Cd137, ILA, Ly63, Secreted CD137 antigen, Tnfrsf9 , Tumor necrosis factor receptor superfamily member 9 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Leu 211 |
| Accession | NP_001070976.1 |
| Calculated Molecular Weight | 47.0 kDa |
| Tag | C-hFc |
| Bio-activity | Immobilized mouse His-TNFSF9 at 10 μg/ml (100 μl/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.