Recombinant Human PRCP Protein (His Tag)
SKU: PKSH032721-50
Recombinant Human PRCP Protein (His Tag)
| SKU # | PKSH032721 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Angiotensinase C, Lysosomal Carboxypeptidase C, Lysosomal Pro-X Carboxypeptidase, PCP, PRCP, Proline Carboxypeptidase, Prolylcarboxypeptidase |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Leu22-His496 |
| Accession | P42785 |
| Calculated Molecular Weight | 54.5 kDa |
| Observed Molecular Weight | 60-90 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM NaAc-HAc, 150mM NaCl, 10% Glycerol, pH4.5. |
| Reconstitution | Not Applicable |
Background
Lysosomal Pro-X Carboxypeptidase (PRCP) belongs to the peptidase S28 family. PRCP is detected in many tissues, with highest levels observed in placenta, lung, and liver. It is also present in the heart, brain, pancreas, and kidney. PRCP exists as a homodimer. PRCP cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. PRCP has been shown to be an activator of the cell matrix-associated prekallikrein.