Recombinant Human PAFAH1B2/PAFAHB Protein (His Tag)
SKU: PKSH032847-50
Recombinant Human PAFAH1B2/PAFAHB Protein (His Tag)
| SKU # | PKSH032847 |
| Expression Host | E.coli |
Description
| Synonyms | PAF Acetylhydrolase 30 kDa Subunit, PAF-AH 30 kDa Subunit, PAF-AH Subunit Beta, PAFAH Subunit Beta, PAFAH1B2, PAFAHB, Platelet-Activating Factor Acetylhydrolase IB Subunit Beta |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser2-Ala229 |
| Accession | P68402 |
| Calculated Molecular Weight | 26.6 kDa |
| Observed Molecular Weight | 31 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Not Applicable |
Background
Platelet-Activating Factor Acetylhydrolase IB Subunit β (PAFAHB) is a cytoplasmic hydrolase. PAFAHB is a member of the GDSL lipolytic enzyme family. It also belongs to Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma), PAFAHB is a catalytic subunit. PAFAHB inactivates PAF by removing the acetyl group at the sn-2 position.