Recombinant Human MMP1 Protein (His Tag)
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Recombinant Human MMP1 Protein (His Tag)
| SKU # | PKSH032740 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CLG, Fibroblast Collagenase, Interstitial Collagenase, MMP-1, MMP1, Matrix Metalloproteinase-1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Phe20-Asn469 |
| Accession | P03956 |
| Calculated Molecular Weight | 52.9 kDa |
| Observed Molecular Weight | 56 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 2mM CaCl2, 1mM DTT, 0.05%Brij35, 10% Glycerol, pH 5.0. |
| Reconstitution | Not Applicable |
Background
Matrix Metalloproteinase-1 (MMP-1) is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 contains several distinct domains: a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin like) domain. MMP-1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, α1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL1, IGFBP3, IGFBP5, pro-MMP2, and pro-MMP9. A significant role of MMP1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into 3/4, 1/4 fragments. MMP1 may also be involved in enzyme cascades, cytokine regulation and cell surface molecule modulation.