Recombinant Human LMAN2/VIP36 Protein (Human Cells, His Tag)
SKU: PKSH033330-50
Recombinant Human LMAN2/VIP36 Protein (Human Cells, His Tag)
| SKU # | PKSH033330 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | C5orf8, Glycoprotein GP36b, LMAN2, Lectin Mannose-Binding 2, VIP36, Vesicular Integral-Membrane Protein 36, Vesicular Integral-Membrane Protein VIP36 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asp45-Arg322 |
| Accession | Q12907 |
| Calculated Molecular Weight | 32.7 kDa |
| Observed Molecular Weight | 33 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 10mM GSH, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Vesicular integral-membrane protein VIP36 is also known as Glycoprotein GP36b; Lectin mannose-binding 2; Vesicular integral-membrane protein 36; LMAN2 and C5orf8. LMAN2 is widely expressed and contains one L-type lectin-like domain. LMAN2 binds high mannose type glycoproteins and may facilitate their sorting; trafficking and quality control. LMAN2 plays a role as an intracellular lectin in the early secretory pathway. LMAN2 interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. LMAN2 is also involved in the transport and sorting of glycoproteins carrying high mannose-type glycans.