Recombinant Human Leukocyte Ig-Like Receptor A5/LILRA5/CD85f (C-6His-Avi) Biotinylated
SKU: PKSH034006-100
Recombinant Human Leukocyte Ig-Like Receptor A5/LILRA5/CD85f (C-6His-Avi) Biotinylated
| SKU # | PKSH034006 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD85 antigen-like family member F, CD85f, ILT-11, Immunoglobulin-like transcript 11, LILRA5, LILRB7, LIR-9, Leukocyte immunoglobulin-like receptor 9, Leukocyte immunoglobulin-like receptor subfamily A member 5 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gly42-Arg268 |
| Accession | A6NI73 |
| Calculated Molecular Weight | 27.9 kDa |
| Observed Molecular Weight | 35-45 kDa |
| Tag | C-His-Avi |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5（LILRA5）is a member of the leukocyte immunoglobulin-like receptors (LILR), comprise a family of activating and inhibitory type immunoreceptors. LILRA5 consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail. The ECD contains two Ig-like domains and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain. LILRA5 is expressed by monocytes, macrophages, and neutrophils. Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10. It can be detected in tissues of the hematopoietic system, including bone marrow, spleen, lymph node and peripheral leukocytes. Crosslink of ILT-11 on the surface of monocytes has been shown to induce calcium flux and secretion of several proinflammatory cytokines, which suggests the roles of this protein in triggering innate immune responses.