Recombinant Human HSP27/HSPB1 Protein (His Tag)
SKU: PKSH031656-100
Recombinant Human HSP27/HSPB1 Protein (His Tag)
| SKU # | PKSH031656 |
| Expression Host | E.coli |
Description
| Synonyms | CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Lys205 |
| Accession | NP_001531.1 |
| Calculated Molecular Weight | 28.0 kDa |
| Observed Molecular Weight | 28 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 20mM HEPES, 0.1M KCl, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
HSP27 is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3.