Recombinant Human HMGB1 protein(N-His)(active)
SKU: PKSH034144-100
Recombinant Human HMGB1 protein(N-His)(active)
| SKU # | PKSH034144 |
| Expression Host | E.coli |
Description
| Synonyms | HMG-1, HMG1, HMG3, SBP-1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Glu 215 |
| Accession | P09429 |
| Calculated Molecular Weight | 25.7 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TNF alpha in RAW264.7 cells.The ED50 for this effect is <10 μg/mL. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
High mobility group protein B1 is a member of the HMGB family consisting of three members; HMGB1; HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein; HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile; it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte; T cell; dendritic cell activities in inflammatory responses.