Recombinant Human CFL2/cofilin 2/ADF Protein (His Tag)
SKU: PKSH031115-50
Recombinant Human CFL2/cofilin 2/ADF Protein (His Tag)
| SKU # | PKSH031115 |
| Expression Host | E.coli |
Description
| Synonyms | NEM7 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala 2-Leu 166 |
| Accession | Q9Y281-1 |
| Calculated Molecular Weight | 20.4 kDa |
| Observed Molecular Weight | 21 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Cofilin 2 (muscle), also known as CFL2, is a member of cofilin family of the actin-binding protein superfamily. Cofilin2 shows significant homology to the other two members: cofilin 1 and DSTN, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. Cofilin 2 (CFL2) is an important regulator of striated myocyte function. Purified cofilin 2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin2 is not expressed in vegetative cells, but is transiently induced during the aggregation stage of development, whereas cofilin 1 was predominantly expressed in vegetative cells.