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Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)– MSE Supplies LLC

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Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)

SKU: E-BC-K245-S-100

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Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)

SKU # E-BC-K245-S
Detection Instrument Spectrophotometer (660 nm)
Detection method Colorimetric method


Product Details

Properties

Synonyms Pi
Sample Type Serum, plasma, tissue
Sensitivity 0.005 mmol/L
Detection Range 0.005-2.0 mmol/L
Detection Method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 1.300% | Average intra-assay CV: 1%
Other instruments required Water bath, Centrifuge, Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months


Images

A Dede et al investigate the assistance on wheat iron biofortification using endophytic Bacillus altitudinis WR 10. Phosphorus (Pi) level in wheat tissue was determined using phosphorus colorimetric assay kit (E-BC-K245-S).

Group NC Spraying Soaking
KPS 43.5 ± 8.29 54.1 ± 11.02a 50.65 ± 7.35a
TGW (g) 49.48 ± 1.01 50.50 ± 1.38 50.08 ± 0.67
N (Mg/g) 37.58 ± 0.75 60.90 ± 1.02a  58.85 ± 0.39a
P (Mg/g) 4.81 ± 0.06 5.06 ± 0.04 4.83 ± 0.01

 

No significant change was observed in wheat tissue.

 

Detection Principle

Inorganic phosphorus react with molybdic acid to produce phosphomolybdic acid. Phosphomolybdic acid can be reduced to molybdenum blue under the action of reducing agent. And the molybdenum blue have a maximum absorption peak at 660 nm. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.

 

Kit Components & Storage

Item Component Size 1
(50 assays)
Size 2
(100 assays)
Storage
Reagent 1 Chromogenic Agent A 25 mL × 1 vial 50 mL × 1 vial 2-8℃, 12 months
Reagent 2 Chromogenic Agent B Powder × 2 vials Powder × 4 vials 2-8°C, 12 months, shading light
Reagent 3 Chromogenic Agent C Powder × 1 vial Powder × 2 vials 2-8°C, 12 months, shading light
Reagent 4 Protein Precipitator 30 mL × 1 vial 60 mL × 1 vial 2-8℃, 12 months
Reagent 5 10 mmol/L Phosphorus Standard 1 mL × 1 vial 1 mL × 1 vial 2-8℃, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

 

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.26 1.05 1.65
%CV 1.3 0.9 0.8


Inter-assay Precision

Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (mmol/L) 0.26 1.05 1.65
%CV 1.2 1.4 1.3


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 102%.

Parameters Sample 1 Sample 2 Sample 3
Expected Conc. (mmol/L) 0.55 1.25 1.75
Observed Conc. (mmol/L) 0.5 1.3 1.8
Recovery rate (%) 99 106 101

 

Sensitivity

The analytical sensitivity of the assay is 0.005 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

 

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration
(mmol/L)
0 0.05 0.1 0.2 0.5 1 2
Average OD 0.026 0.065 0.106 0.181 0.424 0.817 1.611
Absoluted OD 0 0.039 0.08 0.155 0.398 0.791 1.585