Phospho-SMAD2/3 (Thr8) Polyclonal Antibody
SKU: E-AB-21040-200
Phospho-SMAD2/3 (Thr8) Polyclonal Antibody
| SKU # | E-AB-21040 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Applications | WB |
Product Details
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8 |
| Abbre | Smad2/3 (phospho Thr8) |
| Synonyms | JV18-1, MAD homolog 2, MADH2, MADH3, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD family member 2, SMAD2, SMAD3, Smad2, hMAD-2, hSMAD2 |
| Swissprot |
Q15796P84022
|
| Calculated MW | 48 kDa |
| Observed MW | 48 kDa |
| Cellular Localization | Cytosol, Nucleus, heteromeric SMAD protein complex, nuclear chromatin, nuclear inner membrane, nucleoplasm, nucleus, Plasma Membrane, Other locations: cytoplasm, receptor complex, SMAD protein complex, transcription factor complex. |
| Tissue Specificity | SMAD2 is Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
Related Reagents
| Applications | Recommended Dilution |
| WB | 1:500-1:2000 |
Background
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.