MSE PRO Testo(Testosterone) ELISA Kit

SKU: EK0064251-48T
Regular price $ 264.95

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MSE PRO™ Testo(Testosterone) ELISA Kit

Specifications:

Product name Testo (Testosterone) ELISA Kit
Reactivity General
Alternative Names T; Testesteron; Testeronun
Assay Type Competitive Inhibition
Sensitivity 0.64 ng/mL
Standard 100 ng/mL
Detection Range 1.56-100 ng/mL
Sample type serum, plasma, other biological fluids
Assay length 2h
Research Area Endocrinology; Reproductive science; Hormone metabolism
Test principle This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Testo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Testo in the samples is then determined by comparing the OD of the samples to the standard curve.