MSE PRO Sheep BNP(Brain Natriuretic Peptide) ELISA Kit
SKU: EK0064804-96T
MSE PRO™ Sheep BNP(Brain Natriuretic Peptide) ELISA Kit
Specifications:
| Product name | Sheep BNP(Brain Natriuretic Peptide) ELISA Kit |
| Reactivity | Sheep |
| Alternative Names | GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32 |
| Assay Type | Sandwich |
| Sensitivity | 13.2 pg/mL |
| Standard | 2000 pg/mL |
| Detection Range | 31.25-2000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Cardiovascular biology; Neuro science; Hormone metabolism |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep BNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep BNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep BNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep BNP in the samples is then determined by comparing the OD of the samples to the standard curve. |