MSE PRO Rat MTL(Motilin) ELISA Kit
SKU: EK0064328-48T
MSE PRO™ Rat MTL(Motilin) ELISA Kit
Specifications:
| Product name | Rat MTL(Motilin) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | MLN; MAP; Promotilin; Motilin-associated peptide |
| Assay Type | Competitive Inhibition |
| Sensitivity | 29.75 pg/mL |
| Standard | 6000 pg/mL |
| Detection Range | 93.75-6000 pg/mL |
| Sample type | Serum, plasma and other biological fluids |
| Assay length | 2h |
| Research Area | Endocrinology; Gastroenterology; Hormone metabolism |
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MTL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MTL in the samples is then determined by comparing the OD of the samples to the standard curve. |