MSE PRO Rat KLb(Klotho Beta) ELISA Kit
SKU: EK0064874-96T
MSE PRO™ Rat KLb(Klotho Beta) ELISA Kit
Specifications:
| Product name | Rat KLb(Klotho Beta) ELISA Kit |
| Reactivity | Rat |
| Alternative Names | Klotho beta-like protein |
| Assay Type | Sandwich |
| Sensitivity | 6 pg/mL |
| Standard | 1000 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Metabolic pathway |
| Test principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KLb in the samples is then determined by comparing the OD of the samples to the standard curve. |